Journal: Acta Pharmaceutica Sinica. B

Article Title: Arsenic trioxide-based nanoparticles for enhanced chemotherapy by activating pyroptosis

doi: 10.1016/j.apsb.2025.08.003

Figure Lengend Snippet: Immunomodulatory effects of AsMn/Dz@BSA-FA. Analysis of the proportion of (A) CD11c + CD86 + cells, (B) CD3 + CD4 + cells, and (C) CD3 + CD8 + cells in tumor tissues of each group of mice using flow cytometry. (D) Immunofluorescence detection of changes in CD86, CD11c, CD8, and CD4 in tumor tissue after treatment with different formulations, Scale bar: 100 μm. (E) Expression levels of CD86, CD8, and CD4 in tumor tissue after treatment with different formulations. Changes in (F) TNF- α and (G)IL-6 levels in tumor tissue after treatment with different formulations. Data are presented as mean ± SD ( n = 5). ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001.

Article Snippet: APC Anti-Mouse CD11c Antibody, PE Anti-Mouse CD86 Antibody, FITC Anti-Mouse CD3 Antibody, APC Anti-Mouse CD4 Antibody, PE Anti-Mouse CD8a Antibody and Purified Anti-Mouse CD16/32 Antibody were from Elabscience (Wuhan, China).

Techniques: Flow Cytometry, Immunofluorescence, Expressing

Journal: Journal of Virology

Article Title: CD8+ T Cell Immunodominance in Lymphocytic Choriomeningitis Virus Infection Is Modified in the Presence of Toll-Like Receptor Agonists

doi: 10.1128/jvi.05996-11

Figure Lengend Snippet: FIG. 1. Immunodominance hierarchies of LCMV-specific CD8 T cells are altered by TLR23-L administration. Mice were injected with 500 PFU LCMV s.c. with pIC (100 g) and pam3cysk4 (20 g) individually or in combination before quantifying T cell responses in the spleen at days 8 (A) and 12 (B). LCMV-specific CD8 T cell responses were estimated for the NP396, NP205, GP33, or GP276 epitope (A and B) using ICS and measuring IFN- production after in vitro restimulation. Dot plots are representative of immunodominance profiles (i), and representative data of one experiment out of three independent trials standard deviations from triplicate animals in each condition are shown (ii). The controls

Article Snippet: T lymphocytes were stained with phycoerythrin (PE)Cy5-conjugated, rat anti-mouse CD8 clone 53-6.7 (Cedarlane) at 4°C and then fixed with 1% paraformaldehyde before adding fluorescein isothiocyanate (FITC)-conjugated anti-IFN- antibody (0.1% saponin) clone XMG1.2 (Cedarlane) overnight at 4°C.

Techniques: Injection, In Vitro

Journal: Journal of Virology

Article Title: CD8+ T Cell Immunodominance in Lymphocytic Choriomeningitis Virus Infection Is Modified in the Presence of Toll-Like Receptor Agonists

doi: 10.1128/jvi.05996-11

Figure Lengend Snippet: FIG. 2. Tetramer analysis of altered immunodominance between NP396 and GP276. Mice were injected with 500 PFU LCMV s.c. as described in Materials and Methods, with or without pIC and pam3cysk4 individually or in combination. (A to C) LCMV-specific CD8 T cell responses in the spleen were estimated at day 12 with tetramer staining for the NP396 or GP276 epitopes. (A) Dot plots show tetramer-positive CD8 cells from a representative mouse. (B) Graphs summarize the data from three experiments (n 3 mice in each trial). (C) Number of CD8 T cells that are tetramer positive for NP396 or GP276 were estimated. Controls (C) represent infected spleens with CD8 labeling to adjust for the compensation. Naïve splenocytes stained with the same tetramers gave similar background data (data not shown). For immunodominance analyses between TLR-L-treated and untreated mice, the condition where NP396 becomes subdominant is depicted by an asterisk.

Article Snippet: T lymphocytes were stained with phycoerythrin (PE)Cy5-conjugated, rat anti-mouse CD8 clone 53-6.7 (Cedarlane) at 4°C and then fixed with 1% paraformaldehyde before adding fluorescein isothiocyanate (FITC)-conjugated anti-IFN- antibody (0.1% saponin) clone XMG1.2 (Cedarlane) overnight at 4°C.

Techniques: Injection, Staining, Infection, Labeling

Journal: Journal of Virology

Article Title: CD8+ T Cell Immunodominance in Lymphocytic Choriomeningitis Virus Infection Is Modified in the Presence of Toll-Like Receptor Agonists

doi: 10.1128/jvi.05996-11

Figure Lengend Snippet: FIG. 7. Analyses of the conditions that favor changing immunodominance hierarchies during viral infection. Mice were injected with 500 PFU LCMV-WE and TLR23L with TLR4-L (10 g) simultaneously or TLR23L 3 days prior to virus infection. For different flank conditions, mice were injected with virus in one flank and TLR23L in another flank. Eight days p.i., LCMV-specific CD8 T cell responses were estimated using ICS. The data are representative of three experiments. For immunodominance analyses of TLR-L-treated and untreated mice, the change in the profile where NP396 becomes subdominant was depicted by an asterisk.

Article Snippet: T lymphocytes were stained with phycoerythrin (PE)Cy5-conjugated, rat anti-mouse CD8 clone 53-6.7 (Cedarlane) at 4°C and then fixed with 1% paraformaldehyde before adding fluorescein isothiocyanate (FITC)-conjugated anti-IFN- antibody (0.1% saponin) clone XMG1.2 (Cedarlane) overnight at 4°C.

Techniques: Infection, Injection, Virus

Journal: JCI Insight

Article Title: Combined single-cell transcriptome and immune repertoire analysis reveals hepatic and renal immune injury by heat stroke

doi: 10.1172/jci.insight.189825

Figure Lengend Snippet: ( A ) The subclustering of CD8 + T cells identified 6 cell subclusters. ( B ) Dot plot illustrating the scaled expression of representative marker genes across the 6 CD8 + T cell subclusters. ( C ) Pie diagrams displaying the proportion of sample group contributions per annotated CD8 + T cell subclusters. ( D ) Relative contribution of the 6 CD8 + cell subclusters in different sample groups. P values were obtained using the 1-way Kruskal-Wallis test with post hoc Dunn’s test. ( E ) Frequencies of HS-associated CD8 + T cells as determined by flow cytometry. P values were obtained using the 1-way Kruskal-Wallis test with post hoc Dunn’s test. ( F ) Dot plot showing the expression of cytotoxic effector features in CD8 + T cell subtypes derived from the HC, HE, and HS groups. ( G ) Bar plot showing the percentage distribution of TCR clonality types among CD8 + T cell subsets. ( H ) Heatmap illustrating the overlap of TCR clonality types among CD8 + T cell subsets. ( I ) Schematic showing the differentiation trajectory among each CD8 + T cell subset. ( J ) Heatmap displaying dynamic changes in gene expression along the pseudotime of CD8 + T cell differentiation trajectory.

Article Snippet: The following antibodies were used: anti-human CD4 (APC/cyanine, 7317417, BioLegend), anti-human CD3 (APC, APC-65151, Proteintech), anti-human GZMA (PE/Cyanine, 7507221, BioLegend), anti-human CD8a (PE, PE-65144, Proteintech), anti-human GZMB (Pacific Blue, 515407, BioLegend), anti-human CD161 (FITC, FITC-65115, Proteintech), anti-human CD127 (BV510, 563086, BD Bioscience), anti-human CD56 (PE, PE-65067, Proteintech), and anti-human CD16 (PerCP-Cy 5.5, 565421, BD Biosciences).

Techniques: Expressing, Marker, Flow Cytometry, Derivative Assay, Gene Expression, Cell Differentiation

Journal: Journal of Extracellular Vesicles

Article Title: Engineered Low‐Endotoxin Bacterial Biomimetic Vesicles for Enhanced Oral Dual‐Antigen Subunit Vaccine Delivery

doi: 10.1002/jev2.70207

Figure Lengend Snippet: GDH‐gD‐Fc‐CSS‐BBV@COS induce cellular immunity in mice. (A) Flow cytometry analysis of the proportions of the CD3 + CD8 + and CD3 + CD4 + T cells in the spleen. (B) Proliferative capacity in vitro of splenic lymphocytes isolated from mice immunized for 21 days. (C) Inguinal lymph nodes isolated from mice of different immunization groups. (D) IL‐4 and IFN‐γ levels in splenic lymphocyte supernatants measured using ELISA. (E) Serum IL‐4 and IFN‐γ levels measured using ELISA.

Article Snippet: PE‐conjugated anti‐mouse CD86 (50‐0862‐U025), PerCP‐Cyanine5.5‐conjugated anti‐mouse CD3, FITC‐conjugated anti‐mouse CD4 and PE‐conjugated anti‐mouse CD8a antibodies were purchased from Tombo Biosciences (San Diego, CA, USA).

Techniques: Flow Cytometry, In Vitro, Isolation, Enzyme-linked Immunosorbent Assay